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How to Use NCBI Blast. The BLAST search tool can be used to identify matches in gene sequences
by comparing the sequence you enter with all recorded sequences in relevant databases.
Follow these steps to submit a search and receive results quickly and easily. You will
need and internet access. Step 1. Open the Basic Local Alignment Search Tool page from
the National Center for Biotechnology Information website by clicking on BLAST in the Popular
Resources menu. Step 2. Choose a BLAST program to run from the Basic BLAST menu; read the
short descriptions of each program to figure out which one you should run. Step 3. On the
program page, select the BLAST database you want to use from the Database pull-down menu
under Choose Search Set. Step 4. Copy and paste the Accession number, GI number, or
FASTA formatted sequence into the large text box under Enter Query Sequence. Many Accession
and FASTA sequences can be located using NCBI’s Entrez Gene site at "ncbi.nlm.nih.gov/sites/entrez?db=gene":http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene.
GI numbers can be found using the Sequence Revision History tool at "ncbi.nlm.nih.gov/sviewer/girevhist.cgi?":http://www.ncbi.nlm.nih.gov/sviewer/girevhist.cgi?.
Step 5. Click on the BLAST button to submit your search; the page will refresh when the
results are ready. Did you know The first isolation of DNA was performed by Swiss biologist
Friedrich Miescher in 1869.