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[♪ music plays throughout ♪] [STEMCELL Technologies]
HetaSep™ can be used to isolate nucleated cells from a whole blood sample
by removing over 95% of the red blood cells.
It works by inducing the red blood cells to aggregate into long chains, or rouleaux,
which increase their sedimentation rate.
Because the nucleated cells are collected in a single fraction,
HetaSep™ is particularly useful if you plan to isolate granulocytes from the blood sample,
including basophils, eosinophils, and neutrophils.
[Technical Video] [A Guide to Isolating Nucleated Cells from Whole Blood using HetaSep™]
The following technical guide will help you prepare a sample using HetaSep™.
Add 1 part HetaSep™ solution to 5 parts blood.
And mix well by pipetting.
Allow the sample to settle until the sedimented red blood cells
represent approximately 50% of the total volume.
Alternatively you can centrifuge the sample to accelerate the sedimentation process;
make sure the brake is off.
Please refer to the HetaSep™ product information sheet
at stemcell.com for centrifuge times and speeds,
as the results for sedimentation vary with the volume and age of the blood sample.
After centrifugation allow the sample to sit for 10 minutes.
Next harvest the supernatant containing the nucleated cells.
Make sure to collect the layer directly above the red blood cell interface.
Try to avoid the red blood cell layer,
but don't worry if you have some red blood cells in your collected fraction.
Finally wash the harvested cells in the appropriate buffer.
The cells are now ready for cell sub-set isolation
or other downstream applications.
For more information, please see the HetaSep™ product information sheet
or visit stemcell.com.
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