Tip:
Highlight text to annotate it
X
(It is only the screen recorder software)
Open Stream Motion software
(just a message from the software)
This is the software that cames with the microscope camera.
Basically we will use the "Acquisition" tab(on the top right), and "Processing".
The microscope must to be already conected.
The basic tools are this small box, called "Camera Control" that you can see at the main window.
If you can see, try to do like the video shows.
You can see the lens menu, it is very important for calibration. If you choose the wrong lens, the results will be wrong.
Now start seeing the image from microscope. Click em "live" to see the image.
Wait a little bit to see the image
There is the manual and automatic "Exposure" mode, let in the automatic, if you need to do fine-tuning, put on the manual mode. To get homogenous images remember the exposure time and take all pictures with the same exposure time.
(Controling the microscope)
(ROI means Region of Interest)
White balance in ROI(region of interest) is to change the bright depending in which part of the image you are more interested.
So click where you want to see better and wait for the results.
To take a picture click on "Snap". The image will appear in a tab.
It is possible to save the image with a right click on the tab. Choose the folder, the file name and the format (TIFF is the best because you don´t lost information, JPG it is smaller but you lost some information)
Clink in "Live" again to continue using the microscope.
(moving the sample in the microscope)
"Snap" to take a picture.
Live to continue. (moving on microscope)
Snap again.
The calibration needs to know which lens is been used. So if you choose the wrong lens the results will be wrong.
In the bottom of the image you can see which lens were used(the user must select the lens). So the measure results are based on that information.
Now, go to the other tab "Processing".
There is some shape tools, that you can use.
For example a line, that you can measure the length of some object in the image. The result appear in micrometers.
The "Magic Wand" the find particles in the image, and gives the area, very usefull!
You can export the results to EXCEL.
(Watch the next video) Wagner M. Lopes Júnior wagnermlopesj@gmail.com