Tip:
Highlight text to annotate it
X
This video demonstrates how to use the iMPNplate™-1600.
The iMPNplate™ is specifically configured to facilitate execution of
Standard Method 9221C for the estimation of bacterial density.
The tools you will need to use the iMPNplate™-1600 are
a sterile sample vessel,
modified Colitag™ medium,
the iMPNplate™-1600
and, for easy sealing, a roller.
Consistent with method 9221, the plate consists of five 10mL wells,
five 1.0mL wells,
and five 0.1mL wells.
The iMPNplate™ additionally contains an overflow well that allows it to accommodate a full 100mL water sample.
The plate is designed for use with the Colitag™ and modified Colitag™ methods
and final results can be recorded as the Most Probable Number of coliforms and E. coli per 100mL water sample.
To use the iMPNplate™, first open the aseptic packaging being careful not to touch the top of the plate or insides of the wells
Place the plate on a level surface with the larger overflow well closest to you.
Then pour your Colitag™-water sample mixture into the plate, making sure to fill all the wells completely.
The remainder of the sample may be poured anywhere over the top or directly into the overflow well.
Next, take a piece of the sterile sealing film and remove the first paper tab.
Affix the film to the end of the plate and then remove the large paper tab.
Use the roller to finish sealing the plate across the wells from one end to the other.
Remove the final paper tab and finish sealing the film around the sides.
Your sample is now ready for incubation.
The iMPNplate™ is stackable for easy transfer around the lab and incubation.
Following incubation, invert the plate and score the wells for yellow color and fluorescence.
Yellow color indicates the presence of coliforms
and blue fluorescence when viewed under 365nm UV light indicates the presence of E. coli.
Using the combination of positive wells,
the most probable number of bacteria per 100mL water sample can be read
either directly from Standard Methods Table 9221:IV,
or from the MPN table in the product insert.