Tip:
Highlight text to annotate it
X
This tutorial describes how to order a gene synthesis including sequence editing and GeneOptimizer
online optimization for maximum expression yields.
Begin with the “Enter sequence” tab. Enter the name, select the sequence type, enter
the sequence and select the bio safety level. For further details see the tutorial: “overview
of funtions”
Click the “Edit sequence” tab to define your open reading frame. The ORF function
allows you to either select from a pre-set of detected ORFs or to customize your ORF.
Please see the “Show more” feature for further ORFs. You can also add stop codons
to your sequence or delete them. Your selected ORF will be highlighted in the sequence and
also added to the feature map. The next step is to select your cloning sites. You can either
select from a pre-set of detected sites or add new sites. Please select the 5 prime cloning
site you want to use. Selected sites will remain unchanged during optimization and the
motif will not get generated in the optimized sequence. Select your 3 prime cloning site
accordingly.
Go to the “Optimize sequence” tab and define which expression host the sequence
shall be optimized for. Please note that depending on the expression organism, a feature will
be available for adding the appropriate Kozak sequence. To receive a specialist optimization,
click here. Ensure all sequence editing and specification is complete. Then click the
“Optimize” button to begin online optimization. The duration of optimization will depend on
the sequence length and complexity.
The sequence summary tab will show your sequence optimized for the requested organism. The
sequence can be downloaded in various formats. The summary can be downloaded as PDF. Graphical
data displays the quality of your sequence before and after optimization. Please note,
you can return to the previous tabs to make modifications at any time. Remember to click
the optimize button each time. Once completed click “Save & Close”.
You are now ready to add your project to the cart. View the “Add to cart” tutorial
to see how that process works.