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The DynaMag magnets come in different formats for different needs. They can be used to isolate
pure, viable, and functional cells. We now release the DynaMag-5, which if you are working
with FACS tubes, is the magnet that you want to use.
The magnet holds any tube between 0.5 and 5 mL, working with any size FACS tubes is
possible and easy. The magnet is strong, and the separation is
quick. We still recommend you wait 2 minutes to make sure you capture all of the beads
and cells. Maybe there is some foam at the top of your
sample. This foam might well trap some cells so you want to shake the cells out.
If you use tubes with a cap, you should tilt the whole magnet to make sure you don't lose
any beads and cells that might be in the cap. If you twist the tubes, the beads will form
a tighter pellet and you can move the pellet a little inside the tube. This can be helpful
both for pipetting and when you want to resuspend in a smaller volume later on.
Use your thumb to hold the tube in place when you open the cap. This way you won't disturb
the bead pellet. Pull the cap towards you, and make sure you don't miss out on the few
drops that might be caught in the cap. You can pipet off or even decant the liquid,
whatever you prefer. Just make sure you get rid of all the liquid. If you decant and there
are some droplets left, you can use a small piece of paper to remove them.
Add your buffer, and lift the tube out of the magnet. You can resuspend your cells by
shaking, vortexing or pippeting. Efficient washing is important to get the
best possible purity, here’s a great tip: Twist the tube a couple of times. The beads
and the bound cells will move through the buffer and that’s a great way of washing
them. It's really easy to lift the rack off and
put it back on the magnet, use one hand, or both hands. Notice how the rack clicks into
place. You can buy extra racks as a separate item.
Keep these tips & tricks in mind, and follow the specific protocol supplied with your specific
product. You'll find that isolating pure, viable, and
functional cells is both fast and easy when you use Dynabeads and a DynaMag magnet!