Tip:
Highlight text to annotate it
X
I'm Allison Campbell, I'm the director of EMSL
and I'm here with Si Wu from the mass spectrometry
group and Si can you tell me a little bit about yourself
and where you are from? Yes,
I'm original from China as you can see from my name
and I actually from the
east coast of China random providence
so you drink some beer from a Chinese restaurant you
might know a city called Chengdu. My home town
is kind of close to Chengdu but it is not Chengdu.
So how long have you been in EMSL as a the staff member?
Since 2010. So you have
been in the mass spectrometry
group the whole time? Yeah
I say I'm I've been there since then. So we're in
front of one the high field magnets, what is
it that you do here in the laboratory? Yes, part of
the work I do is top-down
proteomics out what is a top-down proteomics?
It is a way to analyze a protein at the impact level
and a treatment for them So the benefit is a way
to characterize a functional protein.
I think in their active form Not a like in traditional proteomics
where most people will use some enzymes to cut
proteins into pieces like
you're looking at a bunch of proteins although the
pieces don't have any biology like function
for them so that's why I like
it's very hard to analyze protein at the at the impact level.
So while that it is the benefit of this bigger instrument is like
a high sensitivity and high
resolution of the
detection so for this case the researcher will be able to identify
like and characterize the biological protein
So now we can characterize proteins up to several thousand.
So with top-down proteomics we may be able to go
a level further. So in top-down proteomics you are able
to look at the intact protein you're able to maintain biology,
biological information yes and the High field gives you
very good sensitivity. So can you describe a
project or two that
you're working on? Yeah,
Intramural program yes, the opportunity for me is
I got Intramural funding from EMSL a few years ago.
Which is focused on the bacterial glycosylation
characterization. That is
a very good opportunity to combine all the different
capabilities at EMSL. Not only the top-down
proteomics but also the bottom-up proteomics which
we can use some enrichment method to enrich the encapsulated peptide 0:02:54.849,0:02:59.530 to characterize them. At the same time we also
utilize the Mass Spec which is also available
at EMSL to characterize the glycan structure.
We have some software and analysis tools to combine
all of the information together this is to our knowledge
the only wide system level static of the glycoprotein.
So if you have seen these videos you know that the
staff get to ask me any question that they want
and I'll do my best to answer it. So do you
have any question of me that you would like to
have answered? Once that you mentioned that you
are trying to learn Chinese
That's true.
So do you have more opportunity to go to China?
to learn Chinese? Yes
I've been to China a fair amount.
and I really love the country
I love the people that
are there.
and I've been to Chengdu,
Beijing, Shiyan, Bei'an, Shanghai and
Chuzhou.
Chuzhou is very beautiful. Very beautiful but it was
August so it was very hot.
So what whatever country go to I try to learn just a few
phrases so that I can show some respect to that country that I'm in
and so
Hanshi Hu who is a post doc here, I have lunch with her
once a month and she's been trying to teach me some Chinese. Okay
and I've been trying to learn my tones, which is very important
and very difficult.
yeah all yeah I enjoy it and I try to greet the staff and say
and hello in their native language sometimes.
Which is just somthing that I like to do.
That's nice to know yeah
No problem, Thank you very much.