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Good morning I'm Christine Suh welcome to this news briefing from the 246th
national meeting & Exposition
at the American Chemical Society in Indianapolis we're joined today by
Dr. James Carey
from the National University of Kaohsiung
who will give us a brief overview of his research. For those of you joining us
here in person please silence your cell phones and one asking a question
please state your name and affiliation using the microphone provided
We're also joined by reporters online yeah
our ACS lives Ustream channel
our reporters are encouraged to ask questions after the overview
also joining us if you are a ACS
science writers who might have questions but will feel those from news media and
bloggers first
for online media please link to the archived version
of this session which can be found at
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Now I'll turn to Dr. Carey who will talk to us today about a new
artificial nose device that can speed the diagnosis
of sepsis. Good morning today I would tell you about
a new device so we developed that can detect sepsis in human blood
on the current technology involves putting a single sensor
into the bottom of a bottle and when the patient presents a doctor they put
the blood inside the bottle and then they shake it
and then what happens is the sensor changes color
and when it does the doctor knows the patient has bacteremia
So what we've done
is put 36 dyes inside the same bottle
and then we can get identification from the bottle.
Okay do we have any questions?
So what kind of developments did it take to
achieve this new device? Um actually is quite difficult. You think that just
putting up a sensor into a bottle would be easy
but you can't autoclave this because it's reacts with volatile organics from
bacteria
and so the dilemma is how to make a sterile device
with the CSA attached to it and so what we did
was we took a simple bottle and put a PVDF membrane across to it
and so bacteria cannot go this way, OK?
so what you do is you put water inside the bottle and then autoclave it
and then when you're done you dump the water out
and then you'd here a CSA to the outside. Doesn't it make sense?
So basically you don't have to sterilize the array
and so volatile organics can go this way
but bacteria can go either way so
what you do is you incubate this you put the bacteria a blood sample
in an incubator and shake it and then as the bacteria grow they'll release volitile
organics
and the sensor detects those volatile organics. So what takes
many people and many days many steps
we can do in one step. So Insert bacteria in here and just let it go.
can you compare this technology to the current
diagnosis system that we have available?
like yeah just did that basically. If you
the current device you need after you grow it you need to cult...
after cultivation you take it out and do many many biochemical steps
to get identification whereas our bottle you get
detection and identification in single step. And can you compare the
the amount of time that it takes for the different test?
That's really hard to say because some bacteria take 30 days to growth
Fungus are used to 30 days, some bacteria can take 3, 4 days.
Depends on how much bacteria in the blood. It depends a lot of conditions
Those patient have antibiotics in their blood, young patient, old patient how much
blood you've already to get.
So it's really hard directly compare, but in the same time they get detection
we will have identification. Okay
can you wait for the microphone sorry
and please state your name and affiliation.
so you mentioned did you say 36 dyes that you added to the
there are thirty-six dyes but you actually...oops...
there're 36 dyes but you actually get a hundred and eight
different color channels because each dye has red green and blue. So when you look
at my data
you'll see tons of... you'll see on hundred and eight lines coming out.
What is it? Do you get fingerprints for the particular bacteria to tell
what it is that the color
, the sort of color spectrum would correspond to a certain bacteria
is up
what happened exactly so the the power of this array is exposure over time
so you expose just one time for any application
is sometimes difficult to actually most times difficult to get any information
out
but you can expose Israel for long periods of time
and then you get different color changes either negative or positive sometimes
they go posit that they go negative
you get tons a temp world or a lot of a temp or information involve
and therefore you can back out the identity than the other bacteria
so I mean does that correspond to 108 different species of bacteria that you
can identify all know is the mall
yeah it's unlimited how many different bacterial content if I because each
you exactly we said earlier it's like a fingerprint
how does cialis correspond to the smells up to bacteria
yourself a chromatic ties so when they react differently with different
organic volatile organics there's a might help for friends and their salts
metal salts
different dyes actually for the first time ever
I've listed all the formulations for this array my last paper and the analyst
and so if you wanna know the formula exact formulation you got my last paper
the soap
support information so what it does is they change colors response to different
concentrations %uh volatile organic compounds
what actually all the compounds the bacteria admit key set a date certain
classes of compounds on what types of things
I love this question because that's really display the beauty of this a red
is that we don't share what's being involved um
you can see all the mask take aspect papers they care what's being above all
they have to know what's being involved
but we don't have to know
if you're if you're actually curious about which pseudomonas switch Bowl
organics
just go to the you know you can google it and then you'll see five mas pequeña
present identify
what what type of bacteria can you identify me can you identify some
a difficult to identify the mains Ulm
by other means what you mean by that conventional techniques
yes conventional techniques that does nothing to be
antibiotic susceptibility to me very clear this is not going to replace
antibiotic susceptibility so when you grow the bacteria culture bottle
the doctors gonna want them to to culture and to grow into
get a single colony and then to take that colony and to antibiotic
susceptibility
okay then the doctor will get a plea to read out telling them what
antibiotic will kill the bacteria growing this will not do that
all this will do is help direct appropriate antibiotics
in a in a microbial 30 early on during the patient's
subsys remember you can only take up the for sample when they show up you take
the sample
and then that's all you got because they start giving the patient broad spectrum
antibiotics right away
because they know that the identification process could take days
so what actively used to identify every single 1 I've tried
has have been able to identify so that's up to 30 organisms using this
this bottle he gives me examples
um its me if you re my recent papers as nine in there and then
basically we can tell down to the street level to equalize
there's a 3 bacteria that medical device companies use for
I don't for to quality control
and weaved we've detected those two and identified to swing
the caller
unveils the chemistry wealth your particular aspects this workers have
been developing
the diets or developing the mechanism or the interpretation normative
all of the above this array was reported by Kansas like in two thousand
in nature um so the idea was developed a long time ago
we do formulate some dyes specific to what we're looking for
but that's very minimal sorry
do you think had see this as you being depletes
in hospitals TT is it is as simple as
the tapered open in sticking incubated for a day and then
and then somebody will be able to have a look at the top and say right we've got
there
is MRSA whatever it may be ray I believe that that's possible
I'm there's a company out there that's commercializing this device res we speak
so and how stable
of the dice with you have that bottle one that's is a heat-stable can we
send it to you clinics in Africa or is it not to survive the journey
I put media in here the TSB media and also be or Mariel media open their
bottles and put it in here
and is that basically it'll change in the first 10 minutes after that is
stable for up to 60 days at least
so far taking these bottles at 11 airplanes ships in philadelphia ship
them back
filled with media shipped to Philadelphia ship them back and I can
still identify the three quality controls trees that I use
and could just give his native what the cost is compared with
the traditional way identify the Ducks this bothering hear from
Fisher was four dollars so
PVDF membrane is penny maybe and
depends on how much I want to charge for the CSA on makin
if still nothing compared to days have lap time and
yet the other reagents I told my three-year-old son how to identify the
bacteria
so the he did it for free
the these it takes days and lots of money in Lhasa steps that and for
she have child labor laws over here that means that we can't use your
three-year-old
not well we could which or something I
thank you very much yes to have any other questions
red think that's all thank you very much stuff to carry
please join us for an ex press conference at 10:30 on the solar
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