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hi i will talk about the speech unsolicited told deeply attempts
but not
uh... want to remind to boat was progress to reduce that wet explana told
jen expression works
and
if real say is that this is m
piece of d_n_a_ ends this is uh... gene arms is d_n_a_
was a control sequences alone
lady
and said upstream rich in
and to such a control sequences
special
protein brands
such proteins can uh...
burglar corporate things can ways uh...
helped to promote jim expression or
suppressed in the pression
and in are what first experiment we use it
uh... restriction enzyme
to cut
d_n_a_
edsa upstream rich n
at certain places
desist restriction
and um...
rebooked
pieces of jimmy of different plans
and who makes it this proposal
miracle of torah protein
if we know said
xep protein
and we have it in
purify it forum over ten salsa ex trucks
alls of proteins from santa cruz and onto a specific does this unique
sequence
protein woodbine
sold post techniques would work
and three bullets that was jewelry tradition that's it
uh... want peace to reach down protein violence
and
police power second experiment
we use it technique
is uh...
put printing
and
res help or
d_n_a_s
one
we were able to a degrade uh...
bolt
city in the sats was not protected by
protein
and on the protein cog in these ads
world
inside uh...
protein
the list
so we define expat regen of said d_n_a_
uh...
reason reach uh...
active uh... control sequence exist to reach uh...
protein plans
but joe or tradition
and footprint in
uh... don give us information about interaction off key in the and protein
as a more precise close this tool
mess that's only give us uh...
uh...
regen risen
reach uh...
uh...
to reach alone
protein violence
and wasn't is this a region uh... actually
maybe a small
uh... sequence-specific is active uh...
uh... control sequence
and
now iru took uh...
i will talk about not to this experiment
that it'll help us to defy insists uh... region
active sides at this protected by
protein
and i will talk about sir
picture on southwest
so if we need
says assistant piece of d_n_a_
consists as bonds
and fifty-eight them
uprooting
that bond to eat
has a piece of d_n_a_ zip through court after our second experiments
that was integrated to be used in this one
if we'll take a look at this picture we can see is that um...
siskel only etc woltz
to assist role only in a small place
uh... was that this uh...
was a reference to all would d_n_a_ also can be
electric uh...
small
peace of certainty in the pictured a protected by
protein
so
physicists uh...
intolerant friends
piece of d_n_a_ protected by
protein
andthe
by protein and um...
this is kind of the reference tool
small
peas
also on d_n_a_ septic is
rehta consul sequence what does it mean
we can't change any not get dates here
insisted on
region
and pretend steel would be able to bond
solicitous uh...
d_m_e_
but if you'll change
any sequence here
and implemented
this protein within the it will turn violent truces uh...
sequence anymore so really only
missus uh...
quickens as speech only
assists site ends this exercised
on city in the
that this doesn't matter
so-called kindly defines this site
weekend two victories aware
next experiment
so we would be able to attend in the its its peace of
g_m_t_
thrust what to do
we take uh...
use of p_c_r_
making millions of corpus also that
regent bostian these opposed
protected by uh...
protein
and three ect
worded it carefully votes
or what
both today's experiment would be
uh...
modification
interest
difference essays
that means that we have ground to change some uh...
not get that
source not get taxes that is not important
uh...
for
protein binding within and fact protein binding
but souls um...
not get that sets we have a grant to change and that would be important for
protein binding would leave set um... protein for them to be able to buy and
so bum
full our experiment
we are going to treat the power piece of d_n_a_ wisdom is still sulfate
and said
the missile sulfate ward ads
muscle groups
so all colombians but we have learned to use on then he meant it
quantity of off uh... demand still
sulfate
so we expect only
one
missile group to be edit
uh...
d_n_a_
but one um...
teammates that with goat after aurora
p_c_r_
its can be in anyplace where
groaning happens to be on sam
uh... d_n_a_ fragment
and essay sent
if it's going to be
at a place so it is important for
uh... protein binding such
uh... protein wouldn't be able to buy into such m
uh... fragment of d_n_a_
so
next remix uh...
our
d_n_a_ fragments we use protein
and city
protein with brian here
kia souls is uh...
uh... miss awaited components within
uh...
program do you mean by indian
and
say is this one
police wasn't uh...
control regen gazette what programmed bind and of protein
so
we said our next
experiment
we uh... runyan
as a joke recordation
so
on celeste riffraff
lead there
that this
prepare it
different sizes
d_m_e_
surface use it for control
and to know is that um... sis's for example
one hundred too
one hundred basis and this is two hundred
and so on
and uh... physicists alarm
experimental
a family
to read to expect here
uh...
we expect below
two streets two strikes
one would be
uh...
d_n_a_
fragment zipped was retarded
because it have brown
protein attached to it
soul
the molecular weight would be it
much
larger sen uh...
molecule to reach protein does in pune
so we expect it to be somewhere over here
and sys-con to be or what
negative charges side and here
is positive
and
d_n_a_ is
negatively charge it so
it's going to travel to society
and
molecules that uh... doesn't have protein bind to read it
we expect that to be somewhat all here
weekend xd sis
possess brent
from zijo
and ron
call one second experiment
call a second experiment
going to be
uh... rehearsed to 'em
at
purify it
and them
true for request to purifiers is banned and them
we have to treated to me is uh...
protruding
by courier
genital that continue for alone
d_n_a_
it's a place
uh... look to us
missile ated
raised in the social fate so it's cleaves
and
instead of one
d_n_a_ fragment wishes kept to a d_n_a_ fragments
systemic uh...
this is going to be
put a crew will need to know
this is joe uh...
give us uh... disability tool
sees a difference between uh...
molecules
that this different by on that one
not deducted
so each branch here
on celeste uh...
represented different since on that one mopped up tonight
likenesses can be won the bca diet
molecules this is still not clear customs has the story and so on
uh... weeks is that the zoos dental reach protein do you can buy and and we
clear it um...
took to the pieces
now
weaker four only to bend scea
one pieces
smaller
grant to travel
alleged instances and
this piece is
bigger
so it's going to be closer to his eyesight
and
uh... simplify assist
uh... presentation just showroom quake
one to one
going in cuisine xd excited
to reach protein woodlands uh... enabled to brent
but that kind be to also research m boy needs
so uh... as a part of the sheer competing friends
dependent on how many miss so it's a good means recollect
and we'd have to repeats is same experiment
ms other chemicals
was that ordnance early uh... i didn't even
and cytosine
say mean
and sit down
experiment would be
uh... actually
uh... repetition of what right already hit show it to his going in ancestry
rule kept exact sequence for six active site
also protein
so as this is what really matters
for protein uh...
uh... by indians
and them
control
the scope control sequence
to reach separately or go to work for teen violence at helped to promote jim
expression
although suppressant
thank you for your attention that's all for today