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This video will show you how to prepare complete Essential 8™ Medium for the feeder-free
culture of PSCs
Essential 8™ Medium is a fully defined, feeder-free medium formulated for the growth
and expansion of human pluripotent stem cells. It was originally developed as E8 Medium in
the laboratory of James Thomson, and validated by Cellular Dynamics International.
Thaw Essential 8™ Supplement (50X) at 2–8°C overnight. Do not thaw at 37°C.
To prepare 500 mL of complete Essential 8™ Medium, aseptically mix the following components:
DMEM/F-12 (HAM) 1:1 490 mL Essential 8™ Supplement (50X) 10 mL
Complete Essential 8™ Medium can be stored at 2–8°C for up to 2 weeks.
Before using, warm the complete amount of medium required for that day at room temperature
until it’s no longer cool to the touch. Don’t warm the medium at 37°C.