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Hello, and welcome to the advanced InDesign tutorial!
Today we going to learn how to annotate pictures.
We start with this picture over here
yes I know it's again some science stuff
but the techniques we are learning today
you can apply (them) for every picture you want to annotate.
We want to annotate it like this over here
this is our finished product.
And I first going to show you
why we are not using Photoshop for this.
I see it quite often that people try to annotate their pictures
directly inside Photoshop
or even construct whole
picture arrangements inside Photoshop.
The problem with this is
Photoshop isn't really made font this.
If you want to due those things you should use
Illustrator or InDesign.
Photoshop is really only
to edit you pictures, like adjusting colours and so on.
But I just going to show you what happens when you try to annotate
a gel picture in Photoshop.
If you take your type tool and you
write some labeling like "A B C".
And you would position it above your lanes.
And as our picture itself, as it is a
gel picture from an Agarose gel
It doesn't have a very good resolution, so if we zoom in
you can actually see the pixels of the picture itself
because it's really really small.
However Photoshop
applies these pixel also to your text
so if your picture has a low resolution
also the annotations will get the same low resolution.
because this is not a vector based graphic, it's a pixel based graphic.
And this is a big advantage in InDesign and Illustrator
you have a vector based graphic
so if we jump to InDesign and you zoom in on your picture, you will see
you get the same pixels again
because this is our picture, this is how it is.
but then we have the annotation
that is vector-based, so if you print this
you will get a perfectly smooth labeling.
Okay, now you know which programs not to use
and which programs to use.
So lets get started with InDesign to annotate our gel picture.
You can download the documents we are using today on my website
and you find the link to the documents in the YouTube description on this video.
We have a picture in here that we going to annotate today.
And you want to open this with for example Photoshop
or another application to edit pictures.
we start by cropping the part we want to show in our graphic.
And then you need to invert the image.
We also want to rotate it.
And the only thing I going to edit in here further is the contrast (& brightness).
So I bump up the contrast a lot
and put down the brightness a little bit
so we don't louse the bands we see here.
This should be fine.
And then we can save this picture.
Were not going to override the original picture
we going to save it
as a separate picture.
And we put it in "proto_pictures".
We can call this one
PCR_35_edited.TIF.
Remember this is scientific data, and you should never try to edit or cheat something in here
because people are going to find out and you are going to get into a lot of trouble.
Now it's time to get our picture into our InDesign document.
As always there is a sample document that you can use.
It's this one. And we going to place our picture into this document.
Just drag it into InDesign.
And you see now, it's a little bit big
if we put it in our text frame it would be huge.
So I think halve of the size should be fine.
And if you think about it, if you would place two pictures side by side in your text.
Like this.
They should have, lets say a 5 mm distance between them
so we can take a look, how wide is our text frame.
Its 165 mm, so if each picture
is 80 mm wide, we should get a nice spacing.
like this. We can get rid of the other one.
And now we going to take care of this picture, lets zoom in.
And if you double click in here
you can move this picture around, and scale it a little bit.
We to scale it, that we keep this lain here
because this is our negative control.
And then we need to see if this is exactly strait.
Don't look so strait, so
we going to rotate it just a little bit
and this should be fine. We can move it down
Like this.
and crop this a little bit
and this is our basic picture.
Now it's time to get some labeling in here.
And I first going to start with a line.
And I hold the shift key to draw this line strait.
I (will) give this line a thickness of 1 pt.
Select your line and set it to 1 pt in here.
And then you can go to the strokes panel
and we want to have this line a dashed line.
So what will do, it will make a dashed line.
And you want to select *Adjust dashes and gaps* so you get
the last dash the same size as the others.
And now we need to adjust our gaps and dashes.
So this could be for example 4
and maybe this could be 8
lets see if this works. We can zoom in.
And you want to fit your dashes to your wells where you put your PCR product.
So we need to decrease the spacing a little bit.
maybe 2 is better, and also the size of the dash...
so you basically just play around till you get this right.
OK, this should be fine now.
With this settings you get a nice distribution
of your lines over here.
So this is indicating your wells.
Then of cause we need some labeling, for what we see here.
And we going to use a table for this.
so we start with a text frame
and in here we going to place our table.
We want to have "font" as a basic text style in here.
And if you place a table, you should count how many lanes you have.
In this case we have 22 lanes.
So we going to insert a table
that has one row
and 22 columns.
We can click on *OK*.
And now we should define a table style for this table.
We can do this in here. Pick a new Style.
And we call this one "Gel Picture"
We go on edit
and we need to create a new cell style.
And in here we going to set the cell insets to 0 mm.
And in the Strokes and fill menu we going to set it to 0 pt.
You can click on *OK*
and on *OK* again.
Lets apply this style to our table.
Press *cmd* + *a* to select the whole table
and then go on "Gel Picture".
We also need a new paragraph style for this table.
So lets first apply the style "font".
Like this.
And then I would say we choose the font "Verdana".
And we of cause need to reduce the size
maybe 8 pt is lright.
And we going to define this as a new paragraph style.
We call this one "Gel Picture Font"
and apply it to the whole table.
Lets align all of them in the center
and also on the bottom of the table.
Then I just going to add some labeling in here.
We see now our font is still to big.
So we select the whole table again
and reduce the font size.
This should be *OK*.
With those cells, I would say we change the orientation
and to do this, click in the table once, in the cell
press the *shift* key and press the *arrow* key
to just select a single cell.
In here we can change the orientation
and we still need to change the text size
but you also can increase the table size as well.
Like this, this should be fine.
We do the same thing for the other cells.
And we going to set the 2 in subscript.
Actually we can increase the size of this one back to 6 pt.
And now we need to take care of the alignment.
So we select one cell
and align it in the center
and we going to do the same thing
for the other two cells.
And in this case we also want to align it left.
As well for the other two cells.
Lets take a look how our labeling looks like.
Jup this should be fine.
In the next step I would like to add some labeling on our bands we see here.
To do this I want to look this picture
so I can't move it and don't select it if I work in here.
To do this I need to create a new layer
that should be below "Layer 1"
And I going to select this picture
and just move the blue dot down here.
And if I look it now
I can't select it any more.
And then I go back to layer one
because as long as I am in Layer 2 I can't do edits, because Layer 2 is looked.
I want to add some labeling on our bands to say which size they are.
With the line tool I going to point out the bands I want to label.
And I set the thickens of the line to 0.5 pt.
And I also want to label the line down here.
And then I want to create a new text box
and write down the size of this band.
In this case its 400 bp.
And we want to select our paragraph style "Gel Pic Font".
We set the size to 4 pt.
And we also going to label the band below
that is 300 bp in size.
Lets align both of them.
And then we can go on by labeling our bands.
I would like to highlight all double bands
that have 2 strokes here, like this.
And to do this I going to get the type tool.
chose the paragraph style
"font"
and I going to write down a star in here -> *
I make this one bold
And increase the size.
Lets see if this fits.
Maybe this one is a little bit big.
Lets put it back to 10 pt.
And we change the colour
to white.
Now we can create outlines of this one.
by going to *Type* -> *Create outlines*.
And this will give us a little object that we can position much easier.
We going to copy this a few times.
And tho get the spacing between the stars here right.
I going to use the align tool.
We first select all of them an align them on the bottom
and then I make sure that the outer ones are aligned very well.
This should be fine, maybe I still press this again.
And now we going to distribute all of them in the center (equal spacing to each other), like this.
So they fit perfectly under each band.
Lets move them up a little bit.
And I going to delete the stars that do not have double bands.
Also I might want to change the colour of this one, because we see here
this band is different than the other ones.
Lets make this for example Yellow.
And now we have our bands over here labeled, and we also need to delete this ones. Alrightlright.
You might also want to create some boxes.
I just going to do this as an example.
So you can select your (rectangle) tool
and drag a box like this.
And then you just pick a colour for the border, for example green.
And you have a nice box
around a specific band.
We can adjust it a little bit.
This should be fine.
We can also use some arrow to point out that we have some weak bands
on top of here.
And you might remember, we already have arrows that we used
in a few tutorials before.
But I just going to create a new one, normally you would copy them
but I going to create a new one
so you see it again, in case you missed the (original) one.
You can go to the line tool and press the *shift* key to draw a strait line.
You want to have it 10 mm long.
And the line thickness should be 1 pt.
And you want to have an arrow head.
Like this.
Maybe in this case you want to decrease the size by 50%
and set the line with again to 1 pt, because our (picture) is not that big.
And we going to rotate the arrow.
And also we change the colour to white for example.
Lets put the arrow on some of this bands over here.
Like this.
Now our picture is almost done.
We need to release our background.
We can unlock this layer, select the background, and move this one up.
Now you see it's on top, so we need to send it to the back.
with arrange, set to back.
and now it's fine again.
we can select the whole thing.
And press *cmd* + *g* to group it.
Lets zoom out.
And the last thing we need to do
is to create a text box
and to create a caption for our picture
to describe what we are actually seeing.
So we copy this one, delete it, and place it in here.
We chose the paragraph style "font"
press the return key.
And then we going to select our Figure Numbering to get the automatic numbering of our picture.
We give this one a good description.
Maybe something like this, but of cause you would make it a little bit more accurate in a real world example.
And there we have our final picture.
We can now see how it would look in our text.
And as always we need to add some
text wrap on the side.
Yes I think this looks quite OK.
We actually can always link this directly in our text.
So by copying and then go into you text and paste it again.
And then we make this one an anchored object.
Like this it's bound into our text.
The last thing we have to do is, to apply our object style for our picture.
This one will get the spacing between the description and the picture it self right.
So if we click on "Picture" we can apply this object style
and now you see it gets a little bit more condensed.
Alright and there we have our nice labeled picture.
In the next tutorial we going to talk about how to create a book
because if you have a huge document, it might be easier to divide it into different chapters.
So if you want to watch the video you can click over here and watch it.
Until next time, bye bye!